PHYSICOCHEMICAL CHARACTERIZATION OF 99mTECHNITIUM – LUTEOLIN AS RADIOPHARMACEUTICAL PREPARATION FOR ANTIOXIDANT COMPOUNDAbstract
The objective of this study was to formulate luteolin with radioisotope Technetium-99m (99mTc) to be 99mTc-Luteolin as an antioxidant compound. After optimization, this radiopharmaceutical preparation should be examined for its physicochemical characteristics, such as radiochemical purity, stability, lipophilicity, protein plasma binding, electronic charge, and stability test. The formulation was done by mixing the luteolin compound with radioisotope 99mTc by using SnCl2 as a reducing agent. Radiopharmaceutical compound 99mTc-Luteolin was formed, then tested for physicochemical characteristics. The result of radiochemical purity was 98.19% ± 1.30%, Log P 0.95 ± 0.07, protein plasma binding 38.01% ± 4.28%, and had neutral charges, test stability at room temperature showed that the radiopharmaceutical purity value was better than in plasma. The results obtained in this research work indicated 99mTc-Luteolin radiopharmaceutical compounds had good physico-chemical properties and were relatively stable in room temperature than in blood plasma. This optimization condition has the potential to be developed into a radiopharmaceutical kit.
D. Ramdhani, M. E. Sriyani and R. Mustarichie *
Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, Indonesia.
22 November 2018
20 February 2019
28 February 2019
01 August 2019