PHYTOCHEMICAL ANALYSIS AND DETERMINATION OF ANTIMICROBIAL, ANTIOXIDANT AND ANTICANCER ACTIVITY OF THE LEAF ETHANOLIC EXTRACTS OF PIPER SARMENTOSUM ROXB. IN LAPUYAN ZAMBOANGA DEL SUR, PHILIPPINESAbstract
Piper sarmentosum Roxb. belongs to the genus Piper in which traditional application described as remedy for several diseases. Considering its vast potential as effective pharmacological agent, a study was conducted to assess the phytochemical and biological activities of P. sarmentosum leaf extracts, with particular focus on their anti-microbial, anti-oxidant and anticancer properties. Results for antimicrobial test revealed that it has positive activity against the tested microorganisms namely, Bacillus subtilis and Klebsiella pneumonia. In terms of the antioxidant activity of the extracts, using DPPH scavenging assay, it can be observed that P. sarmentosum has an IC50 of 55.25 ppm, suggesting a potentially significant source of antioxidants. The extracts also exhibited a strong cytotoxic activities against three human cancer cell lines; cervical carcinoma (HeLa), breast cancer (MCF-7), and colon adenocarcinoma (HT-29). The antimicrobial and antioxidant activities of the leaf extract could be attributed to the tannins and flavonoids identified using phytochemical analysis together with steroids and alkaloids. Further analysis of the bioactive compounds using GC-MS analysis revealed the presence of phytochemicals that have anticancer potentials of which the abundant contents were Asarone (73.72%), 3 – (4- methoxyphenyl) propionic acid (7.58%), and Phytol (3.67%). The results of the study show that the ethnomedicinal application of Piper species has a pharmacological basis and that phytochemical search of active compounds inspired by the knowledge from ethnomedicinal application could be vital in drug discovery.
J. M. Sakilan, C. G. Demayo * and P. Opanasopit
Department of Biological Sciences, College of Science and Mathematics, Mindanao State University, Iligan Institute of Technology, Iligan City, Philippines.
24 March 2019
07 September 2019
26 November 2019
01 December 2019