PHYTOCHEMICAL ANALYSIS AND DPPH ANTIOXIDANT ACTIVITY OF TWO TRADITIONALLY USED PLANTS OCCURRING AT PURULIA DISTRICT OFWEST BENGAL, INDIAAbstract
The objectives of the present study were to evaluate the preliminary phytochemical analysis (quantitative and qualitative) and DPPH antioxidant activity of two traditionally important plants occurring at Purulia district of West Bengal in India. Qualitative analysis of phytochemicals (flavonoid, alkaloid, terpenoids, saponins, phenol and carbohydrate) and quantitative analysis of total phenolics and flavonoids were done by using suitable test protocols available in the literature. Antioxidant activity was studied through DPPH assay. Qualitative phytotochemical analysis revealed that the methanolic extract exhibit richness in flavonoids, alkaloids, terpenoids, saponins, phenols and carbohydrates in comparison with aqueous extract. Total phenol and flavonoid content shows highest concentration in methanolic part of A. aspera (1.129mg GA EQ/gm, 3.22mg QEQ/gm) and lowest concentration in aqueous part of S cordifolia (.348mg GA EQ/gm, .324mg QEQ/gm). EC50 values of the two plants were 48.06µg/ml in A. aspera and 52.43µg/ml in S. cordifolia. The results conclude that there is positive correlation between total phenol content and DPPH antioxidant activity. The plants could serve as potential source of natural antioxidants.