PHYTOCHEMICAL ANALYSIS AND IN-VITRO ANTI-OXIDANT ACTIVITY OF HYDROALCOHOLIC EXTRACT FROM TENDER COCONUT MESOCARP

Free radicals are responsible for the development of many diseases. Anti-oxidants play an important role in scavenging free radicals. Anti-oxidant activity of herbal extracts and isolated compounds are reported earlier. Pharmaceutical industries are carrying out research in the development of new potent antioxidants. Novel drug development from economically cheapest plant waste might be useful for many pharmaceutical industries. Tender coconut mesocarp (TCM) is one of the cheapest plant materials used by coir industry etc. Aim of the present study is to analyses various phytochemicals present in the hydroalcoholic extract of TCM and evaluate its antioxidant activity in the in-vitro condition. Qualitative analysis of phytoconstituents present in hydroalcoholic extract (HAE) of TCM shows the presence of carbohydrate, phenolic compounds, flavonoids and tannin. Quantitative analysis of phytoconstituents in HAE shows the remarkable amount of phenols, flavonoids, tannin and vitamin C. LCMS analysis of HAE shows the presence of (±) 13-Azaprostanoic acid with maximum ppm (13.58) and 14 other compounds with ppm > 3n were  identified. The IC₅₀ value of HAE to scavenge DPPH, Nitric oxide radical, Superoxide radical and hydroxyl radical has been calculated as 310.1, 289.3, 282.1, 307.6 µg/ml respectively. In conclusion, HAE is a rich source of phytoconstituents and exhibits potent free radical scavenging activity in the in-vitro condition.