PHYTOCHEMICAL ANALYSIS AND ISOLATION OF FLAVONOID COMPOUND FROM METHANOL EXTRACT OF THE LEAVES OF SCURRULA PARASITICA L.Abstract
Scurrula parasitica L. is a parasitic plant, traditionally used by the people of Mizoram for the treatment of various diseases, and locally called Vatesawi. The bioassay-guided purification of the methanol extract of the leaves of Scurrula parasitica L. led to the isolation of 2-(3, 4-dihydroxy phenyl)-3,5,7-trihydroxy-4H-Chromen-4-one. Quercetin is a flavonol that belongs to one of the subclasses of flavonoids. For separation and purification of compounds, thin layer chromatography and column chromatography were performed using the plant extract. Then identification was done by high-performance liquid chromatography (HPLC) with mass spectral analysis, and confirmation of the isolated compound was performed with thin layer chromatography. The results showed that the solvent system of ethyl acetate 7: chloroform 3 had accurate separation, and the fraction no-200 from column chromatography was processed for identification of the compound. Structures were established with the mass spectrum and confirmation of the compound was done by comparing with standard quercetin, and both appeared at the same wavelength of 254nm (RT 2.63).
Laldingngheta Jerry *, Lalnundanga 1 and Vabeiryureilai M
Department of Forestry, Mizoram University, Aizawl, Mizoram, India.
19 September 2020
10 February 2021
24 May 2021
01 September 2021