PHYTOCHEMICAL ANALYSIS AND PHARMACOLOGICAL EVALUATION OF ETHANOL EXTRACT OF FRUITS OF GREWIA TILIAEFOLIA FOR NEPHROPROTECTIVE ACTIVITYAbstract
Aim: The present study was undertaken to screen the phytochemicals present in fruits of Grewia tiliaefolia and to evaluate the potential role of these fruits in the amelioration of cisplatin-induced nephrotoxicity in male Albino rats. Materials and methods: Ethanol extract of Fruits of Grewia tiliaefolia was prepared by hot extraction method. The prepared ethanol extract was subjected to preliminary phytochemical evaluation followed by TLC, HPTLC, HPLC and GC-MS analysis. The nephroprotective potential of the extract was screened at 200 and 400mg/kg b. w. in both curative and prophylactic regimens in male Albino rats. Nephrotoxicity was induced by single intraperitoneal injection of cisplatin at a dose of 5 mg/kg b.w. The nephroprotective activity was assessed by determining serum markers, urinary parameters, lipid peroxidation and antioxidant levels and his to pathological studies in renal tissue. Results: Upon preliminary phytochemical screening, the ethanol extract showed the presence of Proteins, flavonoids, Tannins, Terpenoids. HPTLC analysis resulted in a fingerprint of extract showing the presence of 14 compounds. TLC and HPLC revealed the presence of quercetin, and GC-MS analysis showed the presence of 80 different phytochemicals. Pharmacological studies revealed that administration of extract significantly attenuated the cisplatin- induced nephrotoxicity remarkably by restoring the biochemical and oxidative stress markers in both curative and prophylactic regimens in dose-dependent manner. Conclusion: Thus, the findings of the present study provided the phytochemical profile and validated the ethnomedicinal use of fruits of Grewia tiliaefolia as a renoprotective agent.
Adikay Sreedevi *, Pravallika BV and Kaveripakam Sai Sruthi
Division of Pharmaceutical Chemistry, Institute of Pharmaceutical Technology, Sri Padmavathi Mahila Visvavidyalayam, Tirupati, Andhra Pradesh, India.
19 September 2020
03 January 2021
24 May 2021
01 September 2021