PHYTOCHEMICAL ANALYSIS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF METHANOLIC EXTRACT OF DATURA STRAMONIUM SEEDS
AbstractThe present study was concerned with phytochemical screening of methanolic extract of Datura stramonium seeds and its antioxidant and antimicrobial activities. The methanolic extract was prepared by using Soxhlet apparatus. The result of phytochemical screening of methanolic extract of Datura stramonium seeds shows presence of various phytoconstituents like alkaloids, flavonoids, amino acids, tannins, saponins, carbohydrates and terpenoids. The methanolic extract reduced the concentration of DPPH free radical with an efficacy near to that of standard antioxidant Gallic acid, but less than BHT. The IC50 value for methanolic extract was 0.82mg/ml. The Nitric oxide activity of methanolic extract was also close to the Gallic acid. The IC50 value obtained for the methanolic extract was 0.70mg/ml. The antibacterial activity was detected by agar well diffusion method against Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Staphyloceceus aureus. The zones of inhibitions obtained were recorded and analyzed against standard control of Ampicillin. The methanolic extract showed higher antibacterial activity of 24.0 mm against E.coli and least antibacterial activity of 13mm against P.aeruginosa. The present study suggests that the use of seeds of this plant may be exploited for health supplements.
Article Information
46
3021-26
415
2285
English
Ijpsr
Samier A. Waza , Prashant Anthony and Sikander Dar *
Department of Chemistry, Sam Higginbottom Institute of Agriculture, Technology and Sciences (Formerly AAI-DU), Allahabad, U.P. India
sikanderdar786@gmail.com
04 December, 2014
27 January, 2015
21 May, 2015
10.13040/IJPSR.0975-8232.6(7).3021-26
01 July, 2015