PHYTOCHEMICAL ANALYSIS OF ETHANOLIC EXTRACT OF PROSOPIS CINERARIA PODS BY GC-MSAbstract
This study was aimed to identify the possible bioactive chemical constituents of the state tree of Rajasthan, i.e., Prosopis cineraria L. (locally called Khejri) by GC-MS analysis using the GC-MS equipment (Shimadzu GCMSQP2010). For this purpose, the ethanolic extract of P. cineraria pods were used. Experimental conditions of the GC-MS system were as follows: Rtx-5MS capillary standard non-polar column, dimension: 30m, ID: 0.25 mm, Film: 0.25 µm was used, and flow rate of mobile phase (carrier gas: He) was set at 1.0 ml/min. Crude samples that were dissolved in methanol were run fully at a range of 40-550 m/z, and the results were compared by using NIST Library. GC-MS analysis of an ethanolic extract of pods led to the identification of 32 compounds viz. Bicyclo[3.1.1]hept-3-ene, 4, 6, 6-trimethyl-2-vi (30.47%), 5-Isopropyl-2methylbicyclo [3.1.0] hexan-2-ol(19.38%), (-)-.beta. –Bourbonene (10.62%), 3-O-Methyl- d-glucose (5.04%), Kessane (4.01%), Thunbergol (3.65%), alfa.-Copaene (2.23%). There is not much information available on the phytochemical constituents of P. cineraria pods. This is the first attempt to investigate the GC-MS analysis of the ethanolic extract of this plant.
G. Sharma, Devki, R. Vyas and R. Sisodia *
Radiation Biology Laboratory, Department of Zoology, Centre for Advanced Studies, University of Rajasthan, Jaipur, India.
08 January 2020
18 May 2020
21 May 2020
01 January 2021