PHYTOCHEMICAL AND IN-VITRO ASSESSMENT OF ANTIHISTAMINIC AND ANTICHOLINERGIC ACTIVITY OF LEAVES OF HIBISCUS SABDARIFFA LINN.Abstract
Objective: The aim of the present study was to find out the herbal drug which has potential antihistaminic properties. Studying the in-vitro antagonistic effect of crude extracts on isolated goat tracheal chain preparation for assessment of direct antihistaminic activity using two agonists like histamine and acetylcholine. Methods: The ethanolic extract of leaves of Hibiscus sabdariffa was found quite useful in showing antihistaminic activities when tested on an experimental isolated goat tracheal. They were divided into five groups. Results: PTEE exerted an antagonistic effect on histamine and acetylcholine-induced contraction (P < 0.05). Significance is seen at a dose of 2, 4, 10 mg/ml for histamine and acetylcholine Figure 6.2 and 6.4 in a dose-dependent manner. Histamine antagonistic effect seen as (70.12 ± 1.727, 56.09 ± 1.2, 48.17 ± 1.321) similarly the acetylcholine antagonistic effect seen as (85.60 ± 2.489, 60.20 ± 2.456, 44.00 ± 1.141). Conclusion: The present study a notable contraction produced by histamine at a dose 1.6µg/ml, as 82 mm taken as 100% while notable contraction was produced by acetylcholine at a dose 1.6 µg/ml, as 92 mm taken as 100% were observed.
M. R. M. Usman * and M. K. Kachhava
Department of Pharmacognosy, Smt. Sharadchandrika Suresh Patil College of Pharmacy, Chopda, Maharashtra, India.
09 August 2020
30 November 2020
17 May 2021
01 August 2021