PHYTOCHEMICAL EVALUATION AND HPTLC ANALYSIS OF PHOENIX SYLVESTRIS FRUIT EXTRACT AT TWO RIPENING STAGESAbstract
Phoenix sylvestris Roxb, commonly known as date sugar palm or wild date palm, grows naturally in the tropics and subtropics of the Indian subcontinent. The present study was aimed for phytochemical evaluation and to develop the high performance thin layer chromatography (HPTLC) fingerprint profile of methanolic extract of P. sylvestris at two ripening stages. The powdered plant material was extracted using methanol. Quantitative analysis of phytochemicals and HPTLC fingerprinting analysis were carried out. The quantitative analysis of the phytochemicals revealed the presence of various amounts of total phenolic, total flavonoids and total tannins. These phytochemicals were significantly higher in the unripened stage than ripened stage. The HPTLC fingerprinting analysis was carried for phenolic compounds by using Camag Linomat 5 instrument which revealed the presence of phenolic compounds in two stages of P. sylvestris. HPTLC data revealed that unripened extract has high quality phytochemicals than ripened extract. The phytochemical changes of P. sylvestris fruit ripening clearly explained its growth, development and ripening stages.
Gangaraju Rapaka *, Archana Bollavarapu and Raghavarao Tamanam
Department of Biochemistry, Andhra University, Visakhapatnam, Andhra Pradesh, India.
22 June, 2016
26 August, 2016
13 September, 2016
01 December, 2016