PHYTOCHEMICAL EVALUATION AND HPTLC FINGERPRINT PROFILE OF CISSUS LATIFOLIA LAM. STEM

Cissus latifolia Lam. (Vitaceae) is a woody climber with leaf-opposed tendrils. It is a medicinal plant used in the traditional system of medicine for the treatment of various ailments. The present study focused on the identification and qualitative determination of phytoconstituent types and establishment of the HPTLC fingerprint profile of the hot and cold extracts of C. latifolia. Preliminary phytochemical screening was done to identify the class of compounds present. HPTLC analyses of eight different extracts were carried out with the most suitable mobile phase system using the Camag HPTLC instrument consisting of Linomat- V automated spotter having a 100 μl syringe connected to a nitrogen cylinder, twin-trough developing chamber, scanner-III and viewing cabinet with dual wavelength UV lamps (Camag, Muttenz, Switzerland). Qualitative phytochemical screening revealed the presence of flavonoids, coumarins, tannins, alkaloids, steroids, terpenoids, saponins, quinines, anthraquinones and phenol in the stem of C. latifolia. The HPTLC profiling of eight different extracts showed the presence of alkaloids, flavonoids, phenols, saponins, and tannins with different R_f values. The results of preliminary phytochemical screening and HPTLC fingerprint obtained from this study can be used as a reference for the standardization and quality control of Cissus latifolia stem.