PHYTOCHEMICAL EVALUATION AND STANDARDIZATION OF POLYHERBAL ETHYL ACETATE EXTRACT AND ITS GEL-BASED FORMULATION OBTAINED FROM BERRIES AND FLAXSEED (EAPEG-BF)

Berries, fruits, and flaxseed have nowadays have increased in research studies. The berries such as Rubus idaeus (Red raspberry), Vaccinium macrocarpon (cranberry), Vaccinium angustifolium (blueberry) and Fragaria x ananassa Duch. (strawberry) have health beneficial compounds like anthocyanins, ellagitannins, ellagic acids, polyphenolic compounds and whereas in flaxseed the polyunsaturated fatty acids (PUFAs), α-linolenic acid, omega-3 fatty acids have a potential effect on health. This study aims to evaluate for standardization of polyherbal extract obtained from berries and flaxseed by qualitative phytochemical analysis, physicochemical analysis, Fourier transform infrared spectroscopy, UV analysis, thin layer chromatography. The maceration method is used for performing the extraction and plant-based edible polymer Acrypol 934 P for the preparation of the polyherbal ethyl acetate extract and its gel-based formulation. Different phytochemical tests and physicochemical evaluation procedures were followed for standardization of extract and afterwards gel formulation. The results confirmed the presence of various phytochemicals including flavonoids (rich in content in each), tannins, saponins, cardiac glycosides, phenolics. Different physicochemical studies including viscosity are some extent change due to gelation at room temperature, FTIR characterizes the presence of a particular functional group in the extract. Thin-layer chromato-graphy for the analysis of flavonoids with respective R_f value is determined. The study concludes that the berries and flaxseed have a potential effect on health benefits and they have a high range of flavonoids by phytochemical screening and stability studies are slight change due to conditions of climatic changes at room temperature.