PHYTOCHEMICAL, FT-IR, AND GC-MS ANALYSIS OF STEM AND LEAF OF TILIACORA ACUMINATA (LAN.) HOOK F & THOMAS (MENISPERMACEAE)Abstract
The present study deals with the phytochemical examination of therapeutic importance of Tiliacora acuminata (Lan) Hook. F & Thomas an important medicinal plant. Qualitative phytochemical analysis of the methanol and ethanol extracts prepared from Tiliacora acuminata stem and leaf revealed the presence of alkaloids, anthraquinones, catechins, coumarins, flavonoids, phenols, quinones, saponins, steroids, sugar, glycosides, tannins, and xanthoproteins. The FT-IR spectrum confirmed the presence of alkyl group, methyl group, alcohol group, ethers, esters, carboxylic acid, and anhydrides. The bioactive components of the ethanol extract of Tiliacora acuminata stem and leaf were investigated using Perkin-Elmer Gas Chromatography-Mass Spectrometry (GC-MS), while the mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology (NIST) library. GC-MS analysis of ethanol extract of stem of Tiliacora acuminata revealed the existence of 9,12,15-Octadecatrienoic acid (Z,Z,Z) (85.6%), 9,12,15,Octadecadienoic acid (Z,Z) (7.2%), Asarone (3.5%), Hexa-decanoic acid, ethyl ester (1.6%), Methyl-α-d-ribofuranoside (1.4%) and 9,12,15,Octadecadienoic acid, methyl ester (E,E)- (0.8%) and leaf of Tiliacora acuminata revealed the existence of 3,7,11,15-Tetramethyl-2-hexadecen-1-ol (14.9%), Squalene (14.5%), Phytol (9.5%), Vitamin E (8.3%) and Heptadecane, 2, 6-dimethyl- (7.9%).
A. Nishanthini, V. R. Mohan * and S. Jeeva
Ethnopharmacology Unit, PG & Research Department of Botany, V. O. Chidambaram College, Tuticorin. Tamil Nadu, India.
26 February 2014
09 April 2014
23 May 2014
01 September 2014