PHYTOCHEMICAL INVESTIGATION AND IN-VITRO ANTIMICROBIAL ACTIVITY OF THE ESSENTIAL OIL FROM RHIZOMES OF HEDYCHIUM SPICATUMAbstract
The chemical configuration and antimicrobial activity of Hedychium spicatum rhizome based essential oil was studied, which is also commonly known as Kapoorkachri, grown grown wildlyacross Karnataka Karnataka, India. Thirty-eight compounds were detected from the rhizome based essential oil by GCMS. The main compound was characterized as Ethyl p-methoxy-cinnamate (50.1%), Ethyl cinnamate (26.22%), and Eucalyptol (5. 68%). The anti-bacterial activity was performed by the Broth dilution method. Oil-DMSO solution in seven dilutions was developed starting from 100%, 50%, 25%, 12.5%, 6.25%, 3.12% and 1.56% against five selected human pathogenic organisms which comprised of Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans. Anti-microbial activity against these pathogens were found to be 25%,25%,25%, 50% and 1.56% respectively. The oil showed positive inhibitory effects on organisms compared to the reference standard of Ciprofloxacin and Amphotericin B.
I. Arumugam *, C. Krishnan, S. Ramachandran, D. Krishnan, D. Das and V. Thamankar
The Himalaya Drug Company, Makali, Bangalore, Karnataka, India.
28 January 2020
30 April 2020
11 May 2020
01 February 2021