PHYTOCHEMICAL PROFILE AND IN-VITRO ANTIOXIDANT POTENCIES OF OROXYLUM INDICUM STEM BARK EXTRACT

Background: Phytochemical profile and antioxidant studies are based on exploring plants for their use in the development of novel therapeutic drugs. Oroxylum indicum (OI), a valuable medicinal plant is native to India, found mainly in the Himalayan foothills and the Western Ghats. It has been used by traditional medicinal practitioners for a variety of ailments and is categorized as vulnerable medicinal plant by the government of India. Objectives: The present study was carried out to evaluate the phytochemical profile and antioxidant potential OI stem bark ethanol extracts. Methods: Phytochemical profiling and in-vitro antioxidant potential of stem bark were determined using standard protocols. The crude extract was subjected to LC-MS analysis for the identification of bioactive compounds. Results: The phytochemical analysis revealed the presence of compounds, namely glycosides, alkaloids, flavonoids, and tannins. At various concentrations tested, the increase in the free radical scavenging activity was observed with an increase in the concentration of extracts. The reducing power of the extract increased in a dose-dependent manner with an increase in concentration. The extract exhibited the ability to chelate metal ions as they reduced the concentration of catalyzing transition metal in lipid peroxidation. OI ethanolic extract showed remarkable ability to inhibit peroxidation in linoleic acid. The total antioxidant capacity of the extract increased linearly in a dose-dependent manner expressed as quercetin equivalents. Conclusion: The obtained results in our study may contribute to further standardization, validation, and research in Oroxylum indicum stem bark-based drugs, which are used in traditional and modern phytomedicine.