PHYTOCHEMICAL SCREENING AND IN-VITRO ANTI-CATARACT ACTIVITY ON THE SEED OF CUCUMIS MELO L. ETHANOLIC EXTRACTAbstract
Objective: Evaluation of in-vitro anti-cataract activity on the Cucumis melo L ethanolic extract seeds by glucose-induced cataract in incubated goat lenses. Methods: Anticataract activity is performed by using an isolated goat lens. The goat lens was divided into six groups. Group I: Lens was incubated in artificial aqueous Humor (Glucose 5.5 mM). Group II: Lens was incubated with artificial aqueous humor (Glucose 55 mM). Group III: Lens was incubated in artificial aqueous Humor (Glucose 55 mM) + 40 μg/ml of Ascorbic Acid; Group IV, V, and IV: Lens was incubated in artificial aqueous humor (Glucose 55 Mm) + 20 μg/ml, 40 μg/ml and 60 μg/ml of ethanolic extract of seeds on Cucumis melo L respectively and subjected to photographic evaluation for opacity; The lens was homogenized using tris buffer, and total protein and water-soluble proteins content were determined. Results: Photographic examination of the eyes showed that treatment with ethanolic extracts of the seeds of Cucumis melo L retarded the progression of lens opacification. Vitamin A, Ascorbic acid, β-carotene, Carotenoids, and Flavonoids these phytoconstituents possess antioxidant properties to reduce the development of cataracts. The total and Water soluble protein’s activity is increased in the extract-treated lenses compared with the standard drug ascorbic acid. Conclusion: From this study, we conclude that ethanolic extract of the seeds of Cucumis melo L significantly reduced cataracts at the dose of 80μg/ml in goat lens.
M. Nithya *, R. Manivannan, Jith Joy, B. Saritha, D. Soundar Raja, V. Suriya and S. Uthayanithi
Department of Pharmacology, Excel College of Pharmacy, Komarapalayam, Namakkal, Tamil Nadu, India.
21 January 2023
27 March 2023
25 April 2023
01 September 2023