PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT STUDIES ON ACETONE EXTRACT OF MANILKARA ZAPOTA L. SEEDS
AbstractThe present study was aimed to investigate the phytochemical compounds and antioxidant activity of acetone extract of Manilkara zapota L. seeds. Preliminary phytochemical screening was carried out with the solvents of different polarities and among them acetone extract was selected for further studies. The antioxidant activity of the acetone extract was evaluated by seven different in vitro methods namely: DPPH, FTC, TBA, Metal chelation assay, hydroxyl radical scavenging activity, phosphomolybdenum assay, FRAP. Among them, the acetone extract showed good DPPH radical scavenging activity; IC50 value 380 μg /ml and was compared to the standard α-tocopherol. The amount of lipid peroxidation was high in initial stages when compared to the later stages when assessed using FTC and TBA assay. The seed extract did not show good hydroxyl radical scavenging activity when compared to the other antioxidant evaluation techniques. In conclusion, the results presented here imply that Manilkara zapota seeds could be considered as nutraceutical/functional foods
Article Information
32
2354-2361
578KB
1397
English
IJPSR
C. Mohanapriya*, S. Uma , Rajathi D. Modilal , V. Nithyalakshmi
Assistant Professor, Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Madhurantakam, Kanchipuram- 603308, Tamil Nadu, India
mohanapriya8@gmail.com
25 December, 2013
20 February, 2014
01 May, 2014
http://dx.doi.org/10.13040/IJPSR.0975-8232.5(6).2354-61
01, June 2014