PHYTOCHEMICAL STUDY AND ANTIBACTERIAL, ANTIFUNGAL AND ANTIOXIDANT PROPERTIES OF BRIDELIA FERRUGINEA AND PTELEOPSIS SUBEROSA

The antifungal, antimicrobial, antioxidant and cytotoxic activities of methylene chloride, methanol and hydroethanolic extracts of the leaves of Bridelia ferruginea  and sterm bark of Pteleopsis suberosa  were investigated against six Gram Positive and Gram negative strains bacteria and six species of Aspergilus. The antimicrobial activity was investigatedby the microtest method using p-iodonitrotetrazolium and theantifungal activity by measuringthe mycelial and sporulation inhibition. The phytochemical study was performed on Thin Layer Chromatography and antioxidant activity was assayed using qualitative and quantitative 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging test. The methanolic extract of Pteleopsis suberosa showed better Minimum Inhibitory Concentration against all the tested bacteria. The strongest activity was observed with the methanolic extract of Pteleopsis suberosa against S. aureus meticillin resistant with Minimum Inhibitory Concentration value of 78µg/ml. All extracts showed an interesting sporulation inhibitory percentage up to 60% whereas little to moderate inhibition was obtained against mycelial development (9.18 to 57.53%). The antioxidant activity of extracts increased in dose dependent manner. The methanolic and hydroethanolic extracts were the most active with inhibitory percentage values varying from 86.09 to 100%. In the brine shrimp lethality bioassay, the LC₅₀ values of tested extracts ranged between 3.74 to 45.38 mg/ml. The results obtained indicated that the plant have interesting bioactive principles and support the use of these plants in the treatment of infectious diseases.