PHYTOCONSTITUENTS INVESTIGATION BY LC-MS AND EVALUATION OF ANTI-MICROBIAL AND ANTI-PYRETIC PROPERTIES OF CYNODON DACTYLON

Among numerous species of plants growing in the wild in India, Garike, orDruva or taxonomically the Cynodon dactylon sp. occupies its unique place in the traditions, religions and cultures of different societies. In the present study, Phytochemical screening of methanolic, petroleum ether, ethanolic and aqueous extracts of Cynodon dactylon revealed the presence of 17 different phytoconstituents. Quantitative analysis ofmethanolic extract revealed the concentration of various bioactive constituents.Antimicrobial activity of both Ethanolic and Methanolic extracts of Cynodon dactylon showed good inhibitory effect for all the tested Bacterial and Fungal strains including E. coli, B. subtilis, S. typhimurium, M. luteus, K. pneumoniae, S. aureus, P. vulgaris, P. aeruginosa, C. albicans, A. flavus, A. fumigatus, P. notatum, Sporothrix schenckii, Stachybotrys chartarum and C. neoformans by Agar well method. TLC for all the extracts showed bands in U.V for the presence of Flavonoids, Alkaloids, Phenols and Glycosoids. HPLC analysis of methanolic extract gave peaks at retention time 2.779, 4.115 and 9.840.LC-MS of methanolic extract of Cynodon dactylon showed the presence of eight compounds. H¹-NMR showed groups relating to acyclic aliphatic compounds and α-mono substituted. FTIR analysis of Methanolic, Aqueous and Petroleum Ether extract revealed the presence of 1⁰Amines, Alkanes (CH₃, CH₂ & CH), saturated Aldehydes etc. functional groups.Antipyretic activity of both Ethanolic and Methanolic extracts showed a significant (P<0.05) dose dependent antipyretic effect in yeast induced elevation of body temperature in experimental rats. Thus, the present study concludes the medicinal significance of Cynodon dactylon extracts having potential Antimicrobial and Antipyretic properties which leads to pharmaceutical applications.