PHYTOSYNTHESIS OF SILVER NANOPARTICLES USING FRUIT EXTRACT OF LEEA INDICA (BURM. F.) MERR. AND THEIR ANTIMICROBIAL ACTIVITYAbstract
Green nanotechnology is gaining importance due to the elimination of harmful chemicals and provides a safe, environmental friendly and economic method for synthesis of nanoparticles. In the present investigation fruit extract of Leea indica (Burm.f.) Merr. was used for the synthesis of silver nanoparticles. Aqueous fruit extract were added to 1 mm silver nitrate solution by changing the colour of reaction mixture from colourless to brown colour indicates the formation of silver nanoparticles further, confirmed by characteristic UV-Vis absorption peak at 400 nm. Synthesized nanoparticles were characterized by FTIR, EDAX, AFM and HR-TEM. The FTIR data revealed that bio-molecules involved in the reduction and capping of silver nanoparticles. The morphology of silver nanoparticles was determined by AFM and HR-TEM. The size of nanoparticles ranges between 10-50 nm. The EDX analysis confirmed the purity and elemental nature of silver. Synthesized silver nanoparticles exhibited synergistic antimicrobial activity in combination with antibiotic against Escherichia coli, Salmonella typhi, Staphylococcus aureus and Bacillus subtilis.
Veena K. Rokhade and T.C. Taranath *
P. G. Department of Studies in Botany, Karnatak University, Dharwad, Karnataka, India.
29 August, 2016
14 November, 2016
01 December, 2016
01 March, 2017