POLYPHENOLIC COMPOSITION AND ANTIMICROBIAL POTENTIAL OF METHANOLIC CORIANDER (CORIANDRUM SATIVUM) SEED EXTRACTAbstract
The coriander seedextract in 80% methanol is examined for the polyphenol composition and the antimicrobial potential against pathogenic bacteria Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus pumilus. Gallic acid, caffeic acid, ellagic acid, quercetin and kaempferol are principle polyphenolics identified and quantified in the coriander extract by HPLC. The antimicrobial activity was examined by determining cell damage and measuring cell inhibition zone. The growth inhibition zones observed by agar well diffusion method are 11.93 to 17.27 mm in diameter in presence of coriander extract. Minimum inhibitory concentration of coriander was 4.16 mg/ml for the bacteria tested. Increased release of intracellular nucleotides and proteinaceous materials from the bacterial cells in the presence of methanolic coriander extract suggests that the primary mechanism of action of coriander extract is membrane damage, which leads to cell death. The results obtained herein further encourage the use of coriander in antibacterial formulations due to the fact that polyphenol rich coriander extract could effectively kill pathogenic bacteria related to foodborne diseases.
Anita Dua*, Gaurav Garg , Dinesh Kumar and Ritu Mahajan
Associate Professor, Department of Biochemistry, University College, Kurukshetra University, Kurukshetra-136119, India
21 December, 2013
02 February, 2014
12 May, 2014
01, June 2014