PREPARATION AND EVALUATION OF ISONIAZIDE NANO-CONJUGATES FOR IMPROVING THE THERAPEUTIC EFFICIENCYAbstract
Raise in the multidrug resistance gave challenge to the treatment of tuberculosis with first line medication due to less response. Nanoparticle drug delivery has proven efficiency in improving the therapeutic efficacy of many drugs. Reduction of dosing frequency was achieved by applying drug delivery using Nano medicine and Green Nanotechnology. The present study aimed in the production of such drug conjugates of Isoniazid using interdisciplinary technology of nano medicine and green nanotechnology. Isoniazid Nano-conjugates were produced by simple incubation and formed conjugates were analyzed for the efficiency of the plant extract to conjugate the drug. The therapeutic efficacy of the conjugated drug was evaluated using luciferase Reporter phage assay against M.tuberculosis H37RV. High drug entrapment efficiency of plant extract was achieved, ranging from 85-95% and effective particle sized drug-conjugates, ranging from 246-356nm were formed. The relative light units were also less indicating the persistence of activity of the Isoniazid and the inhibition was 93%. Hence Isoniazid Nano-conjugates have the potential for TB Chemotherapy which could be a cost-effective method for the production of Nanodrugs.
D. Sarvamangala *, P. Nagasejitha. , S. Phrabu Seenivasan , L. Srinivas and U.S.N. Murthy
Department of Biotechnology, GIT, GITAM University, Visakhapatnam, Andhrapradesh, India.
28 June, 2014
28 August, 2014
17 October, 2014
01 February, 2015