PRODUCTION AND CHARACTERIZATION OF L-GLUTAMINASE BY BACILLUS SP.Abstract
L-Glutaminase (EC.188.8.131.52) is an amidohydrolase which catalyses the hydrolytical deamination of L-glutamine resulting in the production of L-glutamic acid and ammonia. L-Glutaminase is gaining marked importance due to its application potential in cancer therapy, food industry and of high value chemicals like theronine. A variety of microorganisms, including bacteria, yeast, and filamentous fungi, from both soil and marine habitat have been reported to produce L-glutaminase .The optimization of L-glutaminase using one of the prominent bacterial isolate (NRBT2822) in batch mode of cultivation showed that a pH of 7.0, temperature of 37°C and agitation rate of 150 rpm are most suitable parameters for maximum enzyme synthesis in presence of 1.0 g/L of L-glutamine as an inducer. The kinetic studies revealed that the Km and Vmax for the enzyme were 66.83 mM and 0.755 mM/min respectively. It was also found that the enzyme showed highest activity at 37oC with a half life of 3 hours.
Swati Sinha and Vinod Kumar Nigam *
Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi, Jharkhand, India.
28 October, 2015
09 December, 2016
22 January, 2016
01 April, 2016