PRODUCTION OF ANTIBIOTIC FROM ACTINOMYCETES ISOLATED FROM NAGPUR REGION AND OPTIMIZATION OF PARAMETERS TO INCREASE THE YIELDAbstract
Actinomycetes species Streptomyces violaceorubidus, strain RC 1796 was isolated from Nagpur region which was identified from MTCC Chandigarh. The inoculum was prepared for fermentation using Streptomyces violaceorubidus RC 1796.Different fermentation parameters were studied to increase the yield which includes types of fermentation media, temperature, pH, agitation condition, time period of fermentation, inoculum size, and effect of different concentrations of fermentation media ingredients. The best media for fermentation was found to be yeast malt extract broth media. The optimum fermentation temperature was 28oC, pH 7, agitation speed 150 rpm; time period of fermentation 7 days, inoculum size 10%, concentration of media ingredients like yeast extract concentration 0.4% and malt extract concentration 1.0%. Analysis for the extracellular or intracellular antibiotic production was carried out and it was found that there was extracellular production of antibiotic. Production of antibiotic was done on large scale using the optimum conditions and by using yeast malt extracts broth for fermentation.
Rita N. Wadetwar*and Arun T. Patil 2
Assistant Professor, J. L. Chaturvedi College of Pharmacy, Electronic Zone Building, Hingna Road, Nagpur 440016, Maharashtra, India
02 April, 2013
20 May, 2013
15 July, 2013
01 August, 2013