PRODUCTION OF EXTRACELLULAR CELLULASE USING BACILLUS SPECIES ISOLATED FROM RED SOIL AND OPTIMIZATION OF CELLULASE ACTIVITYAbstract
Enzymes plays a major role in industries like textile, leather, sugar, paper, rubber, tea etc., Microbes plays important role in enzyme production due to their fast growth rate, easy to manipulate for getting highly stable enzymes through genetic engineering and requires shorter time for production and purification steps. In our present study, we concentrated on the enzymes used in textile industries. Recently, many novel enzymes that prove to be the efficient in the process of desizing, peroxide removal, bio polishing have been developed for textile industries. In commercial point of view, finding of new enzymes with high activity and desired properties that can be potentially used for those processes is a continuous process. Hence, we tried to isolate the cellulolytic bacteria from red soil and produced the enzyme cellulase using specific media. The isolated bacterial species was identified as Bacillus species by morphological and biochemical analysis. Further the cellulase activity was analyzed and the parameters like pH, temperature, substrate concentration, and incubation time were also optimized.
V. Bharathi * and M. Kanaka
PG and Research Department of Biochemistry, S.T.E.T .Women’s College, Mannargudi, Tamilnadu-614001, India
21 April, 2015
29 June, 2015
10 September, 2015
01 November, 2015