PRODUCTION OF PECTINASE BY USING BACILLUS CIRCULANS ISOLATED FROM DUMP YARDS OF VEGETABLE WASTESAbstract
Bacillus circulans strain isolated from dump yards of vegetable wastes was tested for its abilities to hydrolyze the structural polysaccharides. The effect of different production parameters such as pH, temperature, carbon source, nitrogen source (Organic and inorganic), incubation time, inoculum sizes and surfactants on pectinase production by the isolated bacterial strain was studied. The enzyme production was assayed in submerged fermentation (SmF) condition. The maximum pectinase production was observed with Galactose (256 U/ml), Yeast extract (130 U/ml), Ammonium sulphate (72 U/ml), pH 7.0 (236 U/ml), Temperature 40°C (126 U/ml), Tween-80 (144 U/ml), Incubation time 48 hours (166 U/ml) and Inoculum size level 5% (136 U/ml) in the production medium.
E. Venkata Naga Raju* and G. Divakar
Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur-522 510, Andhra Pradesh, India
12 March, 2013
29 April, 2013
21 June, 2013
01 July, 2013