PROTECTION OF BIOMOLECULES AGAINST IN VITRO OXIDATIVE DAMAGE BY THE ANTIOXIDANTS FROM METHANOLIC EXTRACT OF TRIGONELLA FOENUM- GRAECUM SEEDSAbstract
Fenugreek (Trigonella foenum-graecum) seeds were extracted in 80% methanol to examine the content of various potent antioxidant compounds and their influence on in-vitro oxidation of biomolecules i.e. amount. proteins, lipids and DNA. Fenugreek extract had polyphenols (9.47±0.10 mg GAE/g dry seeds) as major antioxidant principle. Gallic acid (170.335µg), caffeic acid (164.550 µg), ellagic acid (184.879 µg) and quercetin (215.814 µg)/g dry weight fenugreek seeds were identified in methanolic extract by HPLC analysis. Other antioxidant compounds ascorbate, tocopherol and riboflavin were present in very low amounts. Antioxidant properties were assayed using DPPH free radical scavenging activity. Fenugreek extract inhibited the metal induced oxidation of proteins and lipids. Presence of the extract could protect DNA against H2O2 -induced oxidative stress. IC50 value of fenugreek seeds was estimated by these mechanisms. The results of this study indicate that polyphenol rich methanolic extract of fenugreek had efficient free radical scavenging, reducing and metal chelating activity to protect biomolecules like proteins, lipids and DNA against oxidative stress.
Anita Dua*, S. Vats , V. Singh and Ritu Mahajan
Associate Professor, Department of Biochemistry, University College, Kurukshetra University, Kurukshetra- 136119, Haryana, India
02 April, 2013
08 May, 2013
26 July, 2013
01 August, 2013