PROTECTIVE EFFECT OF APIGENIN ON 7, 12- DIMETHYLBENZ (A) ANTHRACENE INDUCED GLYCOCONJUGATES IN THE PLASMA AND BUCCAL MUCOSA OF GOLDEN SYRIAN HAMSTERSAbstract
Present study investigated the protective effect of apigenin on cell surface glycoconjugates abnormalities in 7, 12-dimethyldenz(a)anthracene(DMBA) induced hamster buccal pouch carcinogenesis. DMBA painting in the hamster buccal pouch three times per week for 14 weeks developed oral squamous cell carcinoma. The levels of glycoconjugates (Protein bound hexose, Hexosamine, Lipid bound sialic acid, Total sialic acid and Fucose) were analyzed by using specific colorimetric methods. The levels of glycoconjugates were significantly increased both in plasma and buccal mucosa in tumor bearing hamsters as compared to normal hamsters. Oral administration of apigenin at a dose of 2.5mg/kg bw significantly restored the status of glycoconjugates in DMBA treated hamsters. Our results suggest that apigenin protected cell surface glycoconjugates abnormalities during DMBA induced oral carcinogenesis.
Simon Silvan, Shanmugam Manoharan*, Nagarethinam Baskaran, Sekar Karthikeyan and Murugaraj Manoj Prabhakar
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar – 608002,Cuddalore District, Tamil Nadu, India
02 April, 2011
28 April, 2011
26 June, 2011
01 July, 2011