PROTECTIVE EFFECT OF D-PINITOL ISOLATED FROM AERIAL PARTS OF SOYBEAN PLANTS ON HAEMATOLOGICAL PROFILE AGAINST DOXORUBICIN INDUCED CYTO-TOXICITY IN MICEAbstract
The aim of this research was to examine the protective effect of D-Pinitol on haematological profile against Doxorubicin-induced cytotoxicityin mice. Doxorubicin is a chemotherapeutic agent valuable in treating various cancers regardless of its toxicity to normal cells, including hematopoietic suppression. Oxidative stress and generation of reactive oxygen species appear to be the reason for Doxorubicin-induced cytotoxicity in bone marrow cells. D-Pinitol is a richly available carbohydrate in Soybean plants and it has been shown to possess free radical scavenging activity and antioxidant effect in previous researches. Sixty Swiss Albino mice were divided into ten groups: Control, Doxorubicin (5 mg/kg), D-Pinitol (100 mg/kg, 200 mg/kg, 300 mg/kg and 400 mg/kg) and D-Pinitol (100 mg/kg, 200 mg/kg, 300 mg/kg and 400 mg/kg) + Doxorubicin (5 mg/kg). Blood samples were taken from the mice to determine the blood levels of haematological parameters such as white blood cells, lymphocytes, monocytes, red blood cells, hemoglobin, hematocrit and platelets. The D-Pinitol-only treated groups reported no significant variations in haematological parameters compared to the control group. Theblood levels of haematological parameters were decreased significantly in Doxorubicin alone treated group compared to the control group. In the Doxorubicin and D-Pinitol co-treated groups, D-Pinitol significantly elevated the blood levels of haemato-logical parameters in a dose-dependent manner compared to the Doxorubicin group. Our findings suggested that the D-Pinitolco-treatment with Doxorubicin could effectively alleviate the accompanying Doxorubicin-induced cytotoxicity in bone marrow cells by attenuating oxidative stress and reactive oxygen species production through its antioxidant effect and free radical scavenging activity.
M. Sudha * and T. Vetrichelvan
Department of Pharmacology, Adhiparasakthi College of Pharmacy, Melmaruvathur, Tamil Nadu, India.
18 February 2021
03 April 2021
23 April 2021
01 May 2021