PROTECTIVE EFFICACY OF ALLIUM SATIVUM ON DELTAMETHRIN INDUCED TOXICITY IN REPRODUCTIVE TISSUES OF MALE MICEAbstract
Present study was carried out to assess testicular toxicity of pyrethroid insecticide, Deltamethrin (DM) in male Swiss albino mice, Mus musculus and to evaluate protective role of Allium sativum (AS) in alleviating the detrimental effect of DM. Forty male mice were divided into five experimental groups (8 mice per group) and were categorized : Control- Given distilled water and food ad libitum, Vehicle Control (oil)- Given peanut oil, DM treated- Given 1/5th of LD50 (6 mg/kg body weight) of DM dissolved in peanut oil via oral gavage, AS-given Allium sativum only (200 mg/kg body weight), DM + AS- given Deltamethrin along with Allium sativum (6 mg/kg body wt. + 200 mg/kg body wt.). Experiment was conducted for a duration of 45 days. DM caused a significant reduction in body weight, testicular weight, Total Protein, Superoxide Dismutase (SOD), Catalase (CAT), Glutathione (GSH), Glutathione Peroxidase (GPx), Glutathione Reductase (GRx), Glutathione-S- Transferase (GST) and Glycogen content of testis. DM-treated group also showed a significant increase in testicular malondialdehyde (MDA) and Cholesterol concentrations. Conversely, treatment with Allium sativum improved the DM- induced oxidative damage and other evaluated indices of testes. Results indicate that DM exerts significant harmful effects on male mice and concurrent administration of Allium sativum ameliorates the detrimental effects of DM.
K. R. Desai, N. Moid, P. B. Patel and H. N. Highland*
Department of Zoology & Biomedical Technology, School of Sciences, Gujarat University, Ahmedabad, Gujarat, India.
25 August, 2014
26 November, 2014
27 December, 2014
01 April, 2015