PROTECTIVE PROFILE OF HYDNOCARPUS LAURIFOLIA ON STREPTOZOTOCIN INDUCED OXIDATIVE STRESS IN RATS

The present study was aimed to evaluate the anti-oxidative defence system of Hydnocarpus laurifolia in diabetic rats and its potential effect against the β-cell damage induced by oxidative stress. Hydnocarpus laurifolia is a plant which belongs to the family Flacourtiaceae. According to the Indian system of traditional medicine it is used in the treatment of diabetes mellitus. Also literature reveals that the plant possesses anti-inflammatory, anti-leprotic and anti-oxidant property. Type II diabetes was induced in Wistar rats (180-250 gms) by intra peritoneal injection of Streptozotocin (STZ) at 30 mg/kg body weight. Diabetic rats were orally administered with ethylacetate extract of H.laurifolia (EEHL) at two different doses of 100 and 300 mg/kg body weight. Serum glucose and insulin levels were determined. The in vivo anti-oxidant study was determined by evaluating enzymatic anti-oxidants such as Superoxide dismutase (SOD), Lipid peroxidase (LPO) and Catalase (CAT) in hepatic tissue of STZ induced diabetic rats.The MDA levels were depleted and SOD and CAT enzymes were restored in diabetic rats treated with EEHL at the dose of 300 mg/kg body weight. These findings revealed that H.laurifolia seeds possess potent anti-oxidant and anti-lipid peroxidative activity, thus mitigates STZ-induced oxidative stress and exhibits anti-diabetic properties.