QUALITATIVE SCREENINGS OF PHOTOCHEMICAL AND GC-MS ANALYSIS OF CEROPEGIA ATTENUATA- AN ENDANGERED TUBEROUS PLANTAbstract
Ceropegia bulbosa is a medicinal herb, this is useful in curing many disease like kidney stone and deafness. Ethno botanical study showed that the plant has good medicinal value for tribe of Rajasthan the preliminary phytochemical studies of Ceropegia attenuata tuber and leaf extracts revealed the presence of steroids, glycosides, flavonoids alkaloids, saponins, tannins, terpenoids and potassium salt. Gas chromatography mass spectroscopic investigation of methanolic extract of Ceropegia attenuata – a annual land plant is investigated by GCMS technique while the mass spectra of the compounds found in the extract are matched with the standard library of NIST. GC MS analysis revealed the presence of aromatic hydrocarbons, paraffins, fatty acids, sterol, Oleic acid, ketons and diterpenes etc. Flavone 4′-Oh,5-Oh,7-Di-O-Glucoside (12.09%) with RT=31.78 is present in maximum amount , followed by 9,12,15-Octadecatrienoic acid, methyl ester, (Z,Z,Z)- (7.92%) with RT=16.17 in the methanolic extract of leaves of C.attenuata. Steroids like Ergost-5-en-3-ol-(3.0%), Stigmast-5-Dien-3-Ol, (3.Beta.) (5.26%), Lupeol, Globulol and Phytol were also detected. Tetratetracontane (5.13%) was also present in considerable amount. The GC-MS analyses revealed that the methanolic extract is mainly composed of organic compound, and steroids. Maximum area are found for 2H-Azepin-2-one, 3-(dimethylamino)hexahydro-(29.30%),with RT=8.97 followed by 1,3-Propanediol, 2-(hydroxymethyl)-2-nitro- (10.56%)with RT=9.54 in the methanolic extract of tuber of C.bulbosa.
Riddhu Palawat* and Payal Lodha
Plant Pathology Tissue Culture and Biotechnology Laboratory, Department of Botany, University of Rajasthan, Jaipur - 302004, Rajasthan, India
29 November, 2014
16 February, 2015
17 May, 2015
01 July, 2015