QUANTIFICATION OF GALLIC ACID AND ELLAGIC ACID IN ARJUNARISHTA BY VALIDTAED HPTLC DENSITOMETRYAbstract
Arjunarishta, also known as Parthadhyarishta, is a polyherbal hydro alcoholic formulation and is advised as a choice of remedy in cardiovascular disorders. A simple, precise and accurate HPTLC method has been established for the determination of quercetin and rutin in Arjunarishta–T and Arjunarishta-M prepared by traditional and modern methods respectively and also in its marketed formulation. The developed HPTLC method was validated in terms of precision, accuracy, LOD, LOQ, specificity, robustness and ruggedness. The amount of gallic acid in Arjunarishta-T, M and its marketed formulation was found to be 0.0332, 0.0331 and 0.0330% w/w respectively while ellagic acid was found to be 0.0361, 0.0360 and 0.0359% w/w respectively. This is the first report for the quantification of gallic acid and ellagic acid in Arjunarishta by HPTLC. Furthermore, no TLC densitometric methods have been reported for the quantification of gallic acid and ellagic acid from Arjunarishta.
Preeti Tiwari* and Rakesh K. Patel
Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, India
13 March, 2012
18 April, 2012
22 June, 2012
01 July 2012