QUANTIFICATION OF URSOLIC ACID AND MINERALS FROM HEDYOTIS HERBACEA LINN.Abstract
The whole plant of Hedyotis herbacea Linn. which was claimed to have wound healing activity, was evaluated for phytoconstituent ursolic acid determination. The literature review suggests that ursolic acid was scientifically validated for wound healing activity. So the research work aims to screen the various extract of H. herbacea to determine the ursolic rich fraction. So a high-performance thin-layer chromatographic method was developed for quantitative estimation of ursolic acid in H. herbacea family Rubiaceae. The separation was performed on silica gel 60 F254 HPTLC plates using toluene: ethyl acetate: formic acid (7:3:0.1) as mobile phase for elution of markers from the extract. The determination was carried out by using densitometric absorbance-reflection mode at 516 nm for ursolic acid, and also trace elements such as Magnesium, Calcium, Manganese, Potassium, Iron, and zinc which will favor the wound healing activity, were determined by High-performance liquid chromatography.
K. A. Babu *, S. Sivakrishnan and S. Jasemine
College of Pharmacy, Kannur Medical College, Anjarakandy, Kerala, India.
10 July 2020
16 December 2020
08 May 2021
01 July 2021