QUANTIFICATION OF VARIED MIRNAS IN EARLY AND LATE-ONSET PREECLAMPSIA COMPLICATING PREGNANCIES IN SOUTH INDIAN WOMEN- A COHORT STUDYAbstract
Introduction: Preeclampsia (PE) is a common pregnancy-related hypertensive disorder that may result from abnormal placental development and is a major threat to the mother’s and foetus’s life. Methods: Placental miRNAs (miRNAs) profile was investigated using next-generation sequencing for the identification of potential biomarkers playing a role in aberrant placental development and to understand the pathophysiology(space between ‘the’ and ‘pathophysiology’) of PE. Placental tissues were collected from three patient groups (Control [n = 30], Early Onset of Preeclampsia (EOPE) [n = 30], and Late Onset of Preeclampsia (LOPE) [n = 30]) and performed miRNA profilingby Illumina sequencing, downstream analysis for identification, quantification and expression profiling is done by the quantifier.pl, script, and miRDeep2.pl, script. Results: Sequence analysis showed novel miRNAs that were disease-specific and common among all studied groups, suggesting the underlying placental pathologies in EOPE and LOPE. The real-time PCR analysis of 3 miRNAs (miR4743-5p, miR149-5p, and miR331-5p) was done, and observed differential expression in all three patient groups. MiRNA4743-5p was found to be significantly expressed EOPE when compared to control and LOPE samples. Similarly, the expression of miR331-5p was significant in LOPE samples as compared to other groups, and the expression of miR149-5p was significantly observed in the control group. Conclusion: The integrative expression analysis of these 3 miRNAs has given hope for early diagnosis of PE and associated complications in pregnant women. The study explains for the first time the role of microRNA 4743-5p, 149-5p & 331-5p in early and late-onset preeclampsia.
Vinaya Vijayan *, R. Kannan, Y. Subhashini, S. Tarakeswari, B. Ram Reddy, Ramesh Bangaraiahgari, Rajesh Bangaraiahgari, Aparajita D’souza, Aruna Yerra, K. Divya, N. Udaya Kumar and Banala Rajkiran Reddy
Department of Physiology, Apollo Institute of Medical Sciences and Research, Hyderabad, Telangana, India.
05 April 2023
28 May 2023
31 May 2023
01 November 2023