QUANTITATIVE BIOANALYTICAL DERIVATIVE SPECTROSCOPIC STUDIES OF ZOLPIDEM TARTRATE NANOSPHERES

We developed a quantitative, accurate, and cost-effective ultraviolet (UV) spectrophotometric method to estimate zolpidem tartrate nanospheres. These nanospheres were prepared using the solvent evaporation method. X-ray diffraction (XRD) analysis showed peak heights at 19.914, 23.0138, and 40.5246, with values of 225.20, 61.58, and 29.25. Scanning electron microscopy (SEM) confirmed their morphology at magnifications of 50× and 2× µm. Zeta analysis showed a Z- average of 2234d:nm, a peak at 245.6nm, and a zeta potential of – 14.0mV. The nanospheres had an absorbance at 201 nm using the zero-order spectrum method. First-order derivative spectra showed a maximum at 305 nm and a minimum at 280 nm, with absorbance values of 0.9133 and 0.8041. In method I, using bromocresol green, the nanospheres had an absorbance maximum of 0.426 at 429.6 nm and a minimum of 0.786 at 202.2 nm. The area under the curve (AUC) was calculated from 329 nm to 415 nm, with a linearity range of 5-30 µg/mL and R2 = 0.9869. In method II, with bromophenol blue, absorbance maxima were 0.421 at 367.2 nm and 0.886 at 235 nm. The AUC ranged from 279 nm to 329 nm, and linearity was observed from 5 to 30 µg/mL with R2 = 0.9787. In method III, using Sudan Red–III, absorbance maxima were 1.934 at 372.6 nm and 1.3003 at 219.3 nm, with R2 = 0.9894. The AUC was from 275 nm to 330 nm. Validation in human plasma showed linearity from 100 µg/mL to 500 µg/mL, with R2 = 0.9684. Pharmacokinetic studies found a C_max of 357.274 ± 32.57 ng/mL, T_max of 0.8333 hours, AUC_0-t of 623.32 ± 68.55, and AUC_0-α of 656.013 ± 358.640.