QUANTITATIVE DETERMINATION OF DRUGS BY USING CERIUM (IV) AND RHODAMINE B COUPLE: A SPECTROPHOTOMETRIC STUDYAbstract
Simple, sensitive, accurate and precise spectrophotometric methods for quantitative determination of five drugs viz., Tramadol Hydrochloride (TDH) , Dobutamine (DOB), Trimetazidine (TRMZ), Terazocin (TRZ) and Esmolol (ESM) were developed. The method for each drug depends upon oxidation of drugs by Ce (IV) (Excess) and estimating the amount of un reacted Ce (IV) by Rhodamine-B dye at 557 nm. The calibration curves obeyed Beer’s law over the concentration range of 14-130μg ml-1 (TDH), 1-14 μg ml-1 (DOB), 8-120μg ml-1 (TRMZ), 16-130 μg ml-1 (TRZ), and 12-84 μg ml-1 (ESM). The methods have been validated in terms of guidelines of ICH and has been applied to the analysis of pharmaceuticals
M. Sasikala, B. Tirupati and G.Venkateshwarlu *
Department of Chemistry, University college of science, Osmania university, Hyderabad, India.
24 May, 2015
23 July, 2015
30 September, 2015
01 December, 2015