QUANTITATIVE ESTIMATION OF CATECHIN, QUERCETIN AND β-CAROTENE FROM POLYHERBAL FORMULATIONAbstract
An HPTLC method was developed for qualitative and quantitative estimation of catechin, quercetin and β-carotene in the marketed and in house polyherbal formulation Maharishi Amrit Kalash (MAK-5). The method was developed using precoated HPTLC silica gel 60 F254 as stationary phase and toluene: ethyl acetate: formic acid (12:12:2, v/v/v) as mobile phase for catechin and quercetin and petroleum ether (40-600C): acetone (17:3, v/v) for β-carotene. Detection and quantification were performed densitometrically at 200nm for catechin and quercetin and 430 nm for β-carotene. The Rf values of catechin, quercetin and β-carotene are 0.29 ± 0.01, 0.49 ± 0.01 and 0.70 ± 0.01 respectively. The percentage content of catechin, quercetin and β-carotene in marketed and in house polyherbal formulation are 0.223%w/w, 0.099%w/w and 2.313%w/w and 0.114%w/w, 0.141%w/w and 2.486%w/w respectively. Linearity was observed in the concentration range of 100-800 ng/spot for catechin and quercetin and that of 100-600ng/spot for β-carotene. Quantification of catechin, quercetin and β-carotene was found to be achieved by HPTLC fingerprinting from marketed and inhouse polyherbal formulation.
C.R. Kamath and B. Shah*
Department of Chemistry, K.J. Somaiya College of Science and Commerce, Vidyanagar, Vidyavihar, Mumbai-400077, India
14 August, 2014
17 October, 2014
15 December, 2014
01 April, 2015