QUANTITATIVE ESTIMATION OF LEVOFLOXACIN AND ORNIDAZOLE BY UV SPECTROPHOTOMETER: A MIXED HYDROTROPY SOLUBILIZATION APPROCH

Two simple, accurate, novel, safe and precise methods developed for the simultaneous estimation of poorly water-soluble drugs Levofloxacin and Ornidazole in tablet dosage form using 2M sodium acetate and 8M urea solution (50:50% W/W) as mixed hydrotropic solution. Sodium acetate and urea solution did not show any absorbance above 240 nm and thus no interference in the estimation of drugs were seen. LEVO and OZ follows the Beer’s law in the concentration range of 5-25 µg/ml (r²= 0.9997 and 0.9998). Method-A simultaneous equation method employs 287 and 320 nm as two analytical wavelengths, method-B is absorption ratio method, which uses 301 and 320 nm as two analytical wavelengths were used for estimation of LEVO and OZ. The mean percent label claims of tablet dosage were found to be 98.528±0.431 and 97.916±0.732 in method A, 97.586±0.821 and 98.642±0.293 in method B for LEVO and OZ respectively. The standard deviation, coefficient of variance and standard error were obtained for LEVO and OZ was satisfactorily low. The developed methods were validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values therefore the both methods can be used for routine monitoring of LEVO and OZ in industry in the assay of bulk drug and tablets.