QUANTITATIVE ESTIMATION OF QUERCETIN FROM PHOENIX SYLVESTRIS USING VALIDATED HPTLC METHOD

Introduction: Phoenix Sylvestris (L.) Roxb also known as date palm or Indian sugar palm, belongs to the Arecaceae family. In our present study, we used the fruit part (epicarp, endocarp). The fruit is very delicious in taste worldwide. It’s flavonoid part is reported with quercetin but its quantification has not yet been reported. Aim: The study aimed to carried out the Quantitative estimation of quercetin from Phoenix sylvestris using a validated HPTLC method. Method: The chromatographic analysis was carried out with the solvent system toluene: ethyl acetate: formic acid (5:4:1, v/v/v) to confirm the presence of quercetin. The HPTLC method was validated by using aluminium-backed silica gel plates with the same solvent system. Quantitative estimation was done by AUC plot. Results: Total Flavonoid Content of the methanolic extract of Phoenix sylvestris is 0.79 mg QE/g. The HPTLC method was good, accurate and specific. The methanolic extract was found to possess 1.07mg/100g of quercetin. Conclusion: This study discussed the validated HPTLC method which is simple, accurate and sensitive tool for the analysis quercetin in different plant extracts. Quercetin content of P. sylvestris has been established using this method. Further estimation of Quercetin can be carried out from other palm trees in future studies.