REPEATED-DOSE TOXICOLOGICAL STUDIES OF HYDROALCOHOLIC EXTRACT OF LANNEA KERSTINGII ENGL AND K. KRAUSE (ANACARDIACEAE) AND IDENTIFICATION OF TOXICITY MECHANISMSAbstract
Lannea kerstingii Engl. & K. Krause stem bark (Anacardiaceae) is used to treat anaemia, malaria, infections and Burili ulcer. The antioxidant, antihelmintic and trypanocidal properties have been demonstrated using appropriate in vitro and in vivo assays. But the effective doses have demonstrated toxic effects such as a significant decrease of body weight of the experimental animals. This work aimed to evaluate the 60 days repeated-dose toxicity of hydroalcoholic extract from Lannea kerstingii stem bark. After 60 days administration, L. kerstingii induced 50% of rats death; accumulation of gas in the digestive tract and a significant decrease of rats body weight. The determination of the toxic mechanism has shown that L. kerstingii decreased the daily food intake and the intestinal motility. The decrease of the intestinal motility by I.P. administration was higher than by P.O. administration. Naloxone, inhibited the effect of L. kerstingii on the intestinal motility. L. kerstingii stem bark hydroalcoholic extract possess a significant inhibition effect on ileum contraction. Therefore it can be toxic in prolonged use at higher doses and its use should be avoided in cases of constipation.
Aboudoulatif Diallo *, Kwashie Eklu-Gadegbeku , Amegnona Agbonon , Kodjo Aklikokou , Gado Napo-Koura , Edmond Creppy and Messanvi Gbeassor
Department of Toxicology University of Lome (Togo) Faculty of Sciences, University of Lome (Togo) P.O. Box 216 lomé05
18 June, 2014
28 August, 2014
15 November, 2014
01 February, 2015