ROLE OF SPERM NUCLEAR DNA INTEGRITY STATUS AND THE SEMEN QUALITY OF FERTILE AND INFERTILE MENAbstract
The aim of the study is to evaluate the diagnostic DNA profiles of the assessment of semen analysis data with respect to male fertility potential. Seman analysis taken form 50 patients of known fertility and 50 suspended infertility were studied by conventional semen analysis by suitable methods. The acridine orange test was used to assess the integrity of sperm DNA. The data throw doubt upon the validity of an approach based on the number of deviations for the normal standard values defined by the WHO. The alternative approach as specific semen characteristics (particularly morphology) as the major predictor of fertility produced no beneficial results. The means of sperm concentration in fertile males was significantly higher than infertile males (62.7±18.7 million vs. 36.7±25.40 million). Total sperm motility of fertile males also was significantly higher compared to infertile (56.32±0.07% vs. 28.8±10.7%). Normal sperm morphology of fertile males was also significantly higher compared to infertile (24.5±8% vs. 12.87±4.6%). According to these results we can conclude that testing sperm DNA damage is helpful in selection of spermatozoa with the least amount of damage for use in assisted conception.
K. Sudhagar*, R. Balajee and M.S. Dhana Rajan
Nandita’s fertility & Research Centre , 212, McNichols Road, Chetpet, Chennai – 600031, Tamil Nadu, India
06 July, 2013
11 October, 2013
16 November, 2013
01 December 2013