RP-HPLC ANALYSIS OF METFORMIN HYDROCHLORIDE AND VOGLIBOSE AND STUDY OF ITS DIFFERENT ANALYTICAL PARAMETERAbstract
A simple, specific, precise, and efficient method for the Simultaneous estimation of Metformin HCl and Voglibose tablet by a Reverse Phase-High Performance Liquid Chromatography method is developed and validated. Selected mobile phase were in a combination of acetonitrile: buffer pH- 6.5 in the ratio of 62:38. Optimized column is a stainless steel column packed with base octa decylsilyl silica gel of 250X4.6mm and at 254 nm wavelength for metformin and Voglibose detection by Spectrofluorimeter and excitation wavelength at 350nm and emission wavelength at 430nm. In our study, the validation of analytical method for determination of Metformin and voglibose tablet formulation was performed in accordance the parameters including-system suitability, specificity, linearity of response, accuracy, precision (reproducibility & repeatability), robustness (change of wave length±2 nm). The method is validated according to ICH guidelines.
K. Sonia* and K. Prasad Babu
Department of Pharmaceutical Analysis, K.K College of Pharmacy, Chennai, Tamil Nadu, India
05 February, 2013
12 March, 2013
20 May, 2013
01 June, 2013