RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF RANITIDINE HYDROCHLORIDE AND DOMPERIDONE IN COMBINED TABLET DOSAGE FORMAbstract
A simple reverse phase HPLC method was developed for the simultaneous estimation of Ranitidine hydrochloride and Domperidone present in combined tablet dosage forms. Efficient chromatographic separation was achieved on Phenomenax C18 stationary phase (250 X 4.6 mm i. d., 5μ particle size) with simple mobile phase combination of phosphate buffer: acetonitrile: methanol 40: 30: 30 (V/V/V) in an isocratic mode at a flow rate of 1.5 mLmin-1 at 210 nm. The retention times were 2.417 and 7.375 (±0.5) min for Ranitidine hydrochloride and Domperidone respectively. The proposed method has been applied successfully for the simultaneous analysis of Ranitidine hydrochloride and Domperidone in combined tablet dosage form with good accuracy and precision. The method herein described can be employed for quality control and routine analysis of Ranitidine hydrochloride and Domperidone in pharmaceutical dosage form.
Rakesh Kumar Singh *, S Ramakrishna and Pragya Gupta
College of Pharmaceutical Sciences, Mohuda, Berhampur, Orissa, India
07 April, 2010
14 June, 2010
03 July, 2010
01 August, 2010