RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF LOPINAVIR AND RITONAVIR IN COMBINED TABLET DOSAGEFORM AND IN SPIKED HUMAN PLASMAAbstract
A simple, accurate RP-HPLC method has been developed for simultaneous determination of Lopinavir (LPV) and Ritonavir (RTV) from tablet dosage form on reversed phase Brownlee C18 column. The sample was analyzed using acetonitrile: 10 mM ammonium acetate buffer (pH 4.5): Methanol (40:30:30) as a mobile phase at flow rate of 1.0 ml/min and detection at 210 nm using UV detector. The limit of detection (LOD) was found to be 0.061 µg /ml and 0.083 µg /ml and the limit of quantification (LOQ) was found to be 0.184 µg /ml and 0.25 µg /ml for LPV and RTV, respectively. Linearity was observed in the concentration range of 5-35 µg/ml. Both the drugs were spiked in 5% proportion with human plasma, extracted by plasma protein precipitation by using acetonitrile and analyzed by HPLC. The retention time for RTV and LPV was found to be 10.30 and 12.58 min respectively. The % recoveries of RTV and LPV were found to be 86.38 to 93.24% and 88.56 to 92.34% respectively. The LOD was found to be 86.80 ng /ml and 67.86 ng /ml as well as the LOQ was found to be 258.47 ng /ml and 193.75 ng /ml for LPV and RTV, respectively.
R.B. Mardia*, B.N. Suhagia, T.Y. Pasha and S.P. Chauhan
Faculty of Pharmacy, Dharmsinh Desai University , Nadiad, Gujarat, India
19 February, 2014
15 April, 2014
28 June, 2014
01 August, 2014