RP-HPLC METHOD FOR THE DETERMINATION OF 5-FLUROURACIL IN PHARMACEUTICAL FORMULATIONS AND SPIKED HUMAN PLASMAAbstract
A simple, rapid and accurate reverse phase high-performance liquid chromatographic method for the determination of 5-Flurouracil (5-FU) in pharmaceutical formulations and human plasma samples has been developed and validated. The assay of the drug was performed on a CLC C18 (5 μm, 25 cm 4.6 mm i.d.) with UV detection at 266 nm. The mobile phase consisted of methanol-water mixture in the ratio of 98:2, and a flow rate of 1 ml/min was maintained. The standard curve was linear over the range of 0.9-18.4 µg/ml (r2=0.9966). Analytic parameters have been evaluated. Within-day and between-day precision as expressed by relative standard deviation was found to be less than 2%. The method has been applied successfully for the determination of 5-FU in spiked human plasma samples and pharmaceutical formulations. The method will be useful for routine quality control analysis.
Sanjeevaraddi R. Sataraddi and Sharanappa T. Nandibewoor*
P.G. Department of Studies in Chemistry, Karnatak University, Dharwad, Karnataka, India
22 July, 2010
07 October, 2010
05 November, 2010
01 December, 2010