RP- HPLC METHOD FOR THE ESTIMATION OF ESOMEPRAZOLE MAGNESIUM IN BULK AND ITS PHARMACEUTICAL DOSAGE FORMSAbstract
A simple reverse phase HPLC method was developed for the determination of Esomeprazole magnesium present in bulk and pharmaceutical dosage forms. Efficient chromatographic separation was achieved on Kromasil100-C18 stationary phase (250 X 4.6 mm i.d., 5µ particle size) with simple mobile phase combination of acetonitrile: phosphate buffer 55: 45 (V/V) in an isocratic mode at a flow rate of 1.0 mLmin-1 at 301 nm. The retention time was 4.09 min (±0.5). The proposed method has been applied successfully for the analysis of Esomeprazole magnesium either in bulk or pharmaceutical dosage form with good accuracy and precision. The method herein described can be employed for quality control and routine analysis of Esomeprazole magnesium in pharmaceutical dosage form.
Girish G. Rathi, Rakesh Kumar Singh*, Pankaj Singh Patel, Rajan Kumar Singh and Brijesh Kumar
College of Pharmaceutical Sciences, Mohuda, Berhampur, Orissa, India
10 March, 2010
15 April, 2010
19 May, 2010
01 June, 2010