SALICYLIC ACID AND CHITOSAN MEDIATED ABIOTIC STRESS IN CELL SUSPENSION CULTURE OF ANDROGRAPHIS PANICULATA (BURM.F.) NEES. FOR ANDRO-GRAPHOLIDE SYNTHESISAbstract
Quantitative and qualitative improvement of rare and valuable bioactive plant products is promisingly possible using In-vitro cell culture techniques. The present investigation was conducted with the aim to elicit the synthesis ofandrographolide by subjecting cultured cells of Andrographis paniculata to Salicylic acid (SA) and Chitosan mediated abiotic stressCell suspension culture of Andrographis paniculata (Burm. f.) Nees. was treated with salicylic acid and chitosan at late exponential phase. The results were analyzed using High Performance Liquid Chromatography technique. The suspension culture was treated with 0.05mM, 0.5mM and 1.5mM concentrations of salicylic acid for 24, 48 and 72 hrs of treatment duration. The treatment of chitosan was administered to the cell suspension of Andrographis paniculata with the concentrations of 5 mg, 10 mg and 20 mg per 50 ml of suspension culture and each concentration was studied for 24, 48 and 96 hrs of treatment duration. The results revealed that salicylic acid (0.05 mM) for 24 hrs of treatment duration showed as maximum as 18.5 fold increment in andrographolide content (37.0 μg/g).. The chitosan treatment with 20 mg for 48 hrs duration explored highest elicitation of andrographolide (119.0 μg/g, 59.5 fold) compared to the respective control and rest of the treatments. Chitosan mediated elicitation treatment was superior over the treatments of salicylic acid.
Moinuddin M. Ali Vakil* and Vijay D. Mendhulkar
Department of Botany, The Institute of Science, 15, Madam Cama Road, Mumbai- 4000 32, Maharashtra, India
14 April, 2013
10 June, 2013
14 August, 2013
01 September, 2013