SCREENING FOR BIOFILM-FORMING EFFICACY OF CLINICAL ISOLATESAbstract
Seven pathogenic isolates with clinical history were procured from the Department of Microbiology, RMMCH, Annamalai University, Chidambaram, Tamil Nadu. Among them, five isolates (S1-S5) showed multi-drug resistance to the selected antibiotics. Maximum multi-drug resistance was exhibited by four Gram negative isolates (S1, S2, S4 and S5). Initial qualitative analysis of these four isolates for biofilm forming efficacy by the Congo-Red Agar method revealed that three of these isolates (S1, S2 and S4) were biofilm producers. Maximum biofilm formation was exhibited by S4. Further qualitative analysis of biofilm formation by S4 using the Tube Adherence method yielded a positive result. Biofilm forming efficacy of S4 was then quantitatively analyzed by a spectrophotometric assay. An optical density of 0.38 was recorded at a wavelength of 540 nm, which was significantly higher than the O.D. value of the control (0.05), confirming biofilm formation by the isolate.
Roopshali Rakshit, D. Sangeetha * and P. Ramya
Department of Microbiology, Faculty of Science, Annamalai University, Annamalainagar - 608002, Tamil Nadu, India.
25 August 2021
04 October 2021
11 October 2021
01 May 2022