SCREENING OF MICROORGANISMS FROM DIFFERENT SITES OF RESTAURANTS AND DHABASAbstract
A food borne illness or disease is exactly what the term indicates a disease or illness caused by the consumption of contaminated foods or beverages. It would seem rather obvious that a food borne microbial pathogens, or a microbial toxic product, or another poison such as a poisonous chemical that has somehow contaminated the food and beverages leads to one of the many different food borne illness. Many microorganisms present are associated with infection, spoilage and contamination of food in home, restaurants, dhabas and other various sites. We collected and isolated bacterial and fungal strains by various morphological characteristics, biochemical identification etc. Disc diffusion method was used to study antibiotic resistant capability against infectious microorganisms. In the antibiotics Kirby Bauer (disk diffusion) test only 3 tested S. aureus displayed a inhibition zone against some antibiotic, and they resistant against by Amoxicillin, Oxacillin, Ciprofloxin, but sensitive to Doxycycline, Erythromycin. We tested Bacillus subtilis and Pseudomonas also by antibiotic disc diffusion method and observation of investigators in which only few antibiotics showed resistant against isolates. In Bacillus subtilis resistant was observed against antibiotics Oxacillin, Doxycycline and Pseudomonas aeruginosa more of the antibiotics showed positive result like resistant showing Novofloxacin, Aztreonam, Cefotaxine, Cefixine, Cefdinir. Some fungus isolates were also identified and observed and they are very harmful to human health. The study emphasizes on the microorganism which are present in different sites of local market and restaurants due to which the society is suffering with serious health hazards caused by different food products. There is need to study more in a future to decrease food contamination, food spoilage and overall food illness.
Sheetal Gour, Mayuri Khare, Rakesh Kumar Patidar, K.N. Sahare, Swati Bagde, Snehita Chauhan, Shailendra Singh Parihar, Bharti Mahajan and Vinod Singh*
Department of Microbiology, Barkatullah University, Bhopal, Madhya Pradesh, India
12 August, 2013
17 September, 2013
17 December, 2013
01 January, 2014